This tutorial guides you through a BD FACSComp software lyse/no-wash assay setup run. If you are already familiar with previous versions of BD FACSComp software on Mac® OS 9, refer to the BD FACSComp software (version 5.1 or higher) ReadMe file for a description of new features. Additionally, refer to the ReadMe file for important information about entering bead target values. ReadMe files are located in the Documentation folder/Read Me Files folder on your hard drive.

Read Me Files folder This tutorial covers: • Preparing BD Calibrite™ beads • Performing a lyse/no-wash assay setup run • Optimizing settings for biological samples • Using online Help To perform the steps in this tutorial, you will need: • A cytometer and a computer with BD FACSComp software version 5.1 or later • BD Calibrite beads • Optional: biological samples stained with BD Tritest™ or BD Multitest™ reagents, to be used for the optimization steps. Prepare samples as indicated in the

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