ResolveRT’s deconvolution provides powerful algorithms for improving the quality of microscopic images recorded by 3D widefield and confocal microscopes. Two different methods are supported, namely a so-called non-blind and a blind deconvolution method, both based on iterative maximum- likelihood image restoration. In the first case a measured or computed point spread function (PSF) is required. In the second case the PSF is estimated along with the data itself.

The deconvolution documentation is organized as follows: • General remarks about image deconvolution • Data aquisition and sampling rates • Standard deconvolution tutorial • Blind deconvolution tutorial • Bead extraction tutorial • Performance issues and multi-processing The following modules are included: • BeadExtract - obtain a PSF from a bead measurement •
Convolution - convolve two 3D images • CorrectZDrop - corrects attenuation in z-direction • DataPreprocess - background and flatfield correction Deconvolution - the actual deconvolution front-end

Download ResolveRT User’s Guide (amira for microscopy)