If you wish to do your assignments on a PC, you will need either Windows 2000, XP, or
Vista and Microsoft Visual Studio 2005. Note that Windows 98 or Me are not able to run Visual Studio 2005. You should be familiar with the Windows operating system and PCs in general, and be willing and able to figure out the Visual Studio 2005 environment.
The emphasis in class will be on general programming concepts, not on the workings of Visual Studio (often referred to as just VS), and the code you write will be virtually identical to code written by students using a Macintosh.
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FlowJo software is used for the analysis of flow cytometry data. This tutorial will introduce you to FlowJo using a simple experiment as an example. A FITC anti-CD8 reagent is titrated to determine the most economical concentration for staining in future experiments. Table of Contents Introduction Step 1: Load samples into the workspace Step 2: Analyze the control sample in detail Step 3: Copy gates and statistics to all samples Step 4: Verify gates on all samples Step 5: Generate a graphical report Step 6: Generate a table of statistics FlowJo is a product of Tree Star, Inc. www.FlowJo.com USA: (800) 366-6045 International: 1 011 541-201-0022 FlowJo@treestar.com FlowJo was written by Adam Treister and Mario Roederer, based on concepts developed with David Parks, Martin Bigos and Wayne Moore.
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