FlowJo software is used for the analysis of flow cytometry data. This tutorial will introduce you to FlowJo using a simple experiment as an example. A FITC anti-CD8 reagent is titrated to determine the most economical concentration for staining in future experiments. Table of Contents Introduction Step 1: Load samples into the workspace Step 2: Analyze the control sample in detail Step 3: Copy gates and statistics to all samples Step 4: Verify gates on all samples Step 5: Generate a graphical report Step 6: Generate a table of statistics FlowJo is a product of Tree Star, Inc. www.FlowJo.com USA: (800) 366-6045 International: 1 011 541-201-0022 FlowJo@treestar.com FlowJo was written by Adam Treister and Mario Roederer, based on concepts developed with David Parks, Martin Bigos and Wayne Moore.
Read the rest of this entry »

• 0 Comments

FlowJo (Macintosh) Manual for Macintosh Workspace Overview Workspace Overview Adding Samples Groups Batch Analysis Statistics Summary of Drag and Drop Examples Keyword Search and Replace Graph Window Bivariate display Univariate displays Graph Types Bivariate displays Univariate displays Gating Autogating Tool Backgating PolyVariate Display Output SciBook The Layout Editor Copying Graphs Tables Iteration (Batching) Iterating in the Layout Editor Changing the Iteration Value FlowJo (Macintosh) Formulas in Tables Insert Function Text (String) functions:
Read the rest of this entry »

• 0 Comments

FlowJo (Macintosh) Manual for Macintosh - Workspace Overview Workspace Overview Adding Samples Groups Batch Analysis Statistics Summary of Drag and Drop Examples. Keyword Search and 13 Graph Window Bivariate displays Univariate di18 Graph Types Bivariate displays…19 Univariate di19 Gating Autogating Tool Backgating PolyVariate Display Output SciBook The Layout Editor Copying Graphs Tables Iteration (Batching) Iterating in the Layout Editor Changing the Iteration Value Formulas in Tables Insert Function Text (String) functions: Printing Movies Exporting Exporting Histogram Data and Kinetics Data Platforms Kinetics Cell Cycle A57 Derived Parameters Compensation
Read the rest of this entry »

• 0 Comments